Retraction Note: MiR-195-5p inhibits the cell migration and invasion of cervical carcinoma through suppressing ARL2.

The article "MiR-195-5p inhibits the cell migration and invasion of cervical carcinoma through suppressing ARL2", by S.-S. Pan, H.-E. Zhou, H.-Y. Yu, L.-H. Xu, published in Eur Rev Med Pharmacol Sci 2019; 23 (24): 10664-10671-DOI: 10.26355/eurrev_201912_19764-PMID: 31858533 has been retracted by the Authors for the following reasons: The authors found some inaccuracies in the research due to the number of experiments, as well as problems in the editing process of pictures. These errors may mislead readers and affect scientific research in this field. Figures 2D and 5D have also been questioned on PubPeer in April 2023. https://www.europeanreview.org/article/19764 This manuscript has been withdrawn. The Publisher apologizes for any inconvenience this may cause.

TRIM24 aggravates the progression of ovarian cancer through negatively regulating FOXM1 level.

OBJECTIVE: This study aims to uncover the biological functions of the feedback loop tripartite motif-containing 24 (TRIM24)/Forkhead Box M1 (FOXM1) in the pathological progression of ovarian cancer (OC) and the underlying mechanism. PATIENTS AND METHODS: The expression levels of TRIM24 and FOXM1 in OC tissues and cells were determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The potential correlation between TRIM24 level and clinical indexes of OC patients was analyzed. The Kaplan-Meier curves were depicted for evaluating the prognostic potentials of TRIM24 and FOXM1 in OC patients. The regulatory effects of TRIM24 and FOXM1 on proliferative, migratory, and invasive capacities of SKOV3 and OVCAR3 cells were assessed through functional experiments. The rescue experiments were performed to clarify the feedback loop TRIM24/FOXM1 in influencing the progression of OC. RESULTS: TRIM24 was upregulated in OC tissues and cells. The high level of TRIM24 was linked to higher rates of lymphatic and distant metastasis and worse survival in OC patients. The silence of TRIM24 attenuated proliferative, migratory, and invasive capacities of SKOV3 and OVCAR3 cells. FOXM1 level was negatively regulated by TRIM24, which was downregulated in OC. The low level of FOXM1 predicted worse survival in OC patients. Besides, the rescue experiments demonstrated that the feedback loop TRIM24/FOXM1 aggravated the malignant progression of OC. CONCLUSIONS: TRIM24 is upregulated in OC tissues, and closely linked to the occurrence of lymphatic and distant metastasis. Through negatively regulating FOXM1 level, TRIM24 aggravates the progression of OC.

MiR-195-5p inhibits the cell migration and invasion of cervical carcinoma through suppressing ARL2.

OBJECTIVE: MicroRNAs (miRNAs) have great effects on the progression of cervical cancer (CC). This study aimed to investigate the role of miR-195-5p in CC and to explain the regulatory mechanism between ARL2 and miR-195-5p. PATIENTS AND METHODS: Quantitative Real-Time-Polymerase Chain Reaction (qRT-PCR) was used to detect miR-195-5p levels in CC tissues and cell lines. Transwell assays for cell migration and invasion were also performed. A luciferase reporter assay was used to detect the direct target of miR-195-5p. The protein levels of ARL2 were measured by Western blot analysis. RESULTS: In CC tissues and cell lines, miR-195-5p expression was decreased. Downregulation of miR-195-5p was associated with higher FIGO stage, deep stromal invasion, and lymph node metastasis. Moreover, over-expression of miR-195-5p inhibited cell migration and invasion in CC. Furthermore, it was observed that miR-195-5p directly targeted ARL2, which affected the suppressive effect of miR-195-5p in CC. CONCLUSIONS: MiR-195-5p inhibited cell migration and invasion in CC by suppressing ARL2 expression. The miR-195/ARL2 axis may provide a pathway for cell metastasis in CC.

[Application of three-dimensional ultrasound for diagnosis of congenital preauricular fistula].

Objective: To explore the application of real-time three-dimensional ultrasound in locating the course, the brand and the blind end of internal opening of congenital preauricular fistula. Methods: Congenital preauricular fistula patients from Changhai Hospital were studied. All the cases were evaluated using two-dimensional and three-dimensional ultrasound before surgeries. The surgery findings were utilized as the gold standard. The imaging features of 3D ultrasound were described to determine the accuracy of the blind end location, the course of the main fistula tract and the branch tracts. The diagnosis was compared with the surgery findings. Results: In 89 cases, among which there were 21 branch fistulae. There were 68 bend fistulae without branch. The diagnostic accuracy of both two-dimensional ultrasound and three-dimensional ultrasound was 100%. The accuracy in locating the blind end of internal opening, the course of the branch tracts with 3D ultrasound was 92.1%(82/89), 85.7%(18/21) , compared to 80.9%(72/89), 57.1%(12/21) with 2D ultrasound. It was obvious that 3D ultrasound had more advantages. These differences were statistically significant (χ(2)=4.8, P<0.05; χ(2)=4.2, P<0.05). Conclusions: Compared with 2D ultrasound, 3D ultrasound can accurately locate the blind end of internal opening, the course, and the branch of preauricular fistula, clearly display the morphology and the spatial structure of preauricular fistulaand its surroundings. It has high clinical application value in preoperative imaging examination.